Phic31 attb
WebJan 1, 2013 · Background: PhiC31 integrase is a DNA site-specific recombinase integrates DNA into the chromosomes between the two sites of attB and attP. Several pseudo attPs have been identified in mammalian ... WebSequences of PhiC31 and BxB1 attachment sites For each recombinase, the attB and attP attachment sites share a common central dinucleotide sequence (in red) that is used for the strand exchange...
Phic31 attb
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WebJul 25, 2024 · The PhiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage PhiC31. The PhiC31 integrase mediates precise, unidirectional recombination between two att sites named attB and attP. This integrase has been shown to function efficiently in many different cell types as well as in mice [ 12, 13 ]. WebHave a question, comment, or need assistance? Send us a message or call (630) 833-0300. Will call available at our Chicago location Mon-Fri 7:00am–6:00pm and Sat …
WebPlasmid w+attB from Dr. Jeff Sekelsky's lab contains the insert phiC31 attB, hsp70::w. This plasmid is available through Addgene. Image: Illustrated plasmid map in PNG format. GenBank File: Plasmid sequence and annotations. Use text editor or plasmid mapping software to view sequence.
WebThe present invention provides a method for producing single stranded DNA polynucleotides. In particular, the invention provides a method that utilises a DNA minicircle obtained from a parental minicircle plasmid as a template in an enzyme-mediated rolling circle amplification (RCA) reaction to generate a product that can be cleaved to provide … WebPlasmid w+attB from Dr. Jeff Sekelsky's lab contains the insert phiC31 attB, hsp70::w. This plasmid is available through Addgene. Image: Illustrated plasmid map in PNG format. …
WebThe forward and reverse primers will be designed to incorporate attB1 and attB2 sites respectively, to allow clonase-mediated integration of the PCR product into a Gateway entry vector. The primers will be designed to both have a …
WebA method of controlling a genetically-modified plant, comprising (a) providing a genetically-modified plant, whereby cells of said genetically-modified plant contain a heterologous nucleic acid and whereby said genetically-modified plant is inactive with regard to a cellular process of interest, (b) switching on said cellular process of interest by directly … money in antiguaWebJun 12, 2024 · The phage-derived phiC31 integrase is a useful tool for mediating sequence-specific genomic integration in mammalian cells, recombining donor plasmids bearing the attB recognition site with introduced genomic attP sites or endogeneous pseudo- attP sites having partial identity to attP. money in an inspector callsWebPhiC31 Integrase Expression Plasmid. Put the integrase into your PhiC31 and PinPoint genome editing projects with this vector that expresses PhiC31 Integrase for one-step … money in appsWebJul 21, 2006 · The stability of the attB × attP locus, well-documented with the phiC31 integrase system in bacteria and yeast 16,17, was evidenced here by the lack of rearrangement or reversion to the wild-type ... icd 10 code for left hemi hip arthroplastyWebBacteriophage phiC31 encodes an integrase, which acts on the phage and host attachment sites, attP and attB, to form an integrated prophage flanked by attL and attR. icd-10 code for left hemiparesis unspecifiedWebDec 27, 2016 · CHICAGO — If you think your neighborhood has changed since you first moved in, you should see what it looked like 60 years ago. The University of Illinois at … money in and out spreadsheetWebWe use PhiC31 integrase to catalyze recombination between one or two attB sites in a recombinant DNA with three tandem attP sites that were previously inserted into specific loci in the mouse genome (See Figure below). Mouse lines with three tandem attP sites that were knocked into the Hipp11 (H11) or Rosa26 loci (Left in Figure) are zygote donor. icd 10 code for left hand osteopenia